Gliding motility in slide cultures of Myxococcus xanthus in stable and steep chemical gradients.
نویسندگان
چکیده
A method was devised to construct stable and steep chemical gradients in slide cultures to study the movements of gliding cells. The movement of Myxococcus xanthus individual cells and small swarms was studied in these gradients. There was no response to gradients of Casitone and yeast extract that were previously reported to stimulate a positive chemotactic response with M. xanthus.
منابع مشابه
Gliding motility and actinomycin D sensitivity of Fusobacterium nucleatum and other gram-negative rods.
Six strains of gram-negative anaerobic fusiform rods (Fusobacterium and Bacteroides spp.), isolated from deep subgingival locations in humans, were examined for (i) gliding motility in slide cultures, (iii) cell densities on nutrient agar surfaces, and (iii) sensitivity to actinomycin D. Known gliding (FBt) and nonmotile (NM) strains of Myxococcus xanthus served as controls for the gliding and ...
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متن کاملGenetic and molecular analysis of cglB, a gene essential for single-cell gliding in Myxococcus xanthus.
Gliding movements of individual isolated Myxococcus xanthus cells depend on the genes of the A-motility system (agl and cgl genes). Mutants carrying defects in those genes are unable to translocate as isolated cells on solid surfaces. The motility defect of cgl mutants can be transiently restored to wild type by extracellular complementation upon mixing mutant cells with wild-type or other moti...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 178 12 شماره
صفحات -
تاریخ انتشار 1996